Open AccessResponsive fluorinated nanoemulsions for 19F magnetic resonance detection of cellular hypoxia
Author(s) -
Rahul T. Kadakia,
Da Xie,
Hongyu Guo,
Bailey S. Bouley,
Meng Yu,
Emily L. Que
Publication year - 2020
Publication title -
dalton transactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.98
H-Index - 184
eISSN - 1477-9234
pISSN - 1477-9226
DOI - 10.1039/d0dt01182g
Subject(s) - hypoxia (environmental) , chemistry , nuclear magnetic resonance , magnetic resonance imaging , analytical chemistry (journal) , materials science , radiochemistry , nuclear chemistry , nanotechnology , oxygen , physics , organic chemistry , medicine , radiology
We report two highly fluorinated Cu-based imaging agents, CuL1 and CuL2, for detecting cellular hypoxia as nanoemulsion formulations. Both complexes retained their initial quenched 19F MR signals due to paramagnetic Cu2+; however, both complexes displayed a large signal increase when the complex was reduced. DLS studies showed that the CuL1 nanoemulsion (NECuL1) had a hydrodiameter of approximately 100 nm and that it was stable for four weeks post-preparation. Hypoxic cells incubated with NECuL1 showed that 40% of the Cu2+ taken up was reduced in low oxygen environments.