Open AccessResponsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase
Author(s) -
Jerrin Thomas George,
Seergazhi G. Srivatsan
Publication year - 2020
Publication title -
chemical communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.837
H-Index - 333
eISSN - 1364-548X
pISSN - 1359-7345
DOI - 10.1039/d0cc05092j
Subject(s) - nucleotide , fluorescence , transferase , terminal (telecommunication) , chemistry , enzyme , terminal deoxynucleotidyl transferase , biochemistry , rna , combinatorial chemistry , biophysics , biology , computer science , telecommunications , gene , tunel assay , apoptosis , physics , quantum mechanics
We repurposed a terminal uridylyl transferase enzyme to site-specifically label RNA with microenvironment sensing fluorescent nucleotide mimics, which in turn provided direct read-outs to estimate the binding affinities of the enzyme to RNA and nucleotide substrates. This enzyme-probe system provides insights into the catalytic cycle, and can facilitate the development of discovery platforms to identify robust enzyme inhibitors.