Responsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase | Zendy

Jerrin Thomas George | Zendy; Seergazhi G. Srivatsan | Zendy

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Responsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase

Author(s) -

Jerrin Thomas George,

Seergazhi G. Srivatsan

Publication year - 2020

Publication title -

chemical communications

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.837

H-Index - 333

eISSN - 1364-548X

pISSN - 1359-7345

DOI - 10.1039/d0cc05092j

Subject(s) - nucleotide , fluorescence , transferase , terminal (telecommunication) , chemistry , enzyme , terminal deoxynucleotidyl transferase , biochemistry , rna , combinatorial chemistry , biophysics , biology , computer science , telecommunications , gene , tunel assay , apoptosis , physics , quantum mechanics

We repurposed a terminal uridylyl transferase enzyme to site-specifically label RNA with microenvironment sensing fluorescent nucleotide mimics, which in turn provided direct read-outs to estimate the binding affinities of the enzyme to RNA and nucleotide substrates. This enzyme-probe system provides insights into the catalytic cycle, and can facilitate the development of discovery platforms to identify robust enzyme inhibitors.

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