Open AccessIron Fenton oxidation of 2′-deoxyguanosine in physiological bicarbonate buffer yields products consistent with the reactive oxygen species carbonate radical anion not the hydroxyl radical
Author(s) -
Aaron M. Fleming,
Cynthia J. Burrows
Publication year - 2020
Publication title -
chemical communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.837
H-Index - 333
eISSN - 1364-548X
pISSN - 1359-7345
DOI - 10.1039/d0cc04138f
Subject(s) - chemistry , bicarbonate , carbonate , hydroxyl radical , deoxyguanosine , oxygen , ion , reactive oxygen species , buffer (optical fiber) , radical , inorganic chemistry , carbonate ion , photochemistry , organic chemistry , biochemistry , dna , telecommunications , computer science
Product analysis from the iron Fenton oxidation of 2'-deoxyguanosine found reactions in bicarbonate buffer yield 8-oxo-2'-deoxyguanosine and spiroiminodihyantoin consistent with CO3˙-. Reactions in phosphate buffer furnished high yields of sugar oxidation products consistent with HO˙. These observations change the view of DNA oxidation products from the iron-Fenton reaction.