Open AccessNIR-II window tracking of hyperglycemia induced intracerebral hemorrhage in cerebral cavernous malformation deficient mice
Author(s) -
Abdul K. Parchur,
Zhi Fang,
Jaidip Jagtap,
Gayatri Sharma,
Christopher B. Hansen,
Shayan Shafiee,
Wenquan Hu,
Qing Miao,
Amit Joshi
Publication year - 2020
Publication title -
biomaterials science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.422
H-Index - 64
eISSN - 2047-4849
pISSN - 2047-4830
DOI - 10.1039/d0bm00873g
Subject(s) - intracerebral hemorrhage , therapeutic window , medicine , pharmacology , subarachnoid hemorrhage
Second near infrared (NIR-II) window fluorescence imaging between 1000 and 1700 nm with reduced scattering and autofluorescence and deep tissue light penetration allows early and non-invasive determination of vascular pathologies. Here, we demonstrate in vivo NIR-II imaging techniques for tracking hyperglycaemia-induced Intracerebral Hemorrhage (ICH) and Blood Brain Barrier (BBB) hyperpermeability in Cerebral Cavernous Malformation (CCM) deficient mice (CCM1+/-). We synthesised PEGylated Ag 2 S quantum dots (QDs) with a bright fluorescent emission peak centred at 1135 nm under an 808 nm NIR light for dynamic imaging of cerebral vasculature in mice and determined the development of ICH and BBB impairment in hyperglycaemic CCM1+/- mice. In vivo optical imaging was conducted with micro-CT (including k-mean cluster analysis) as well as in vivo permeability assays using FITC-dextran perfusion and IgG staining, respectively. The increased BBB permeability in CCM1+/- mice was further demonstrated to be associated with a high-glucose-caused decrease of CCM1 expressions. This study validates that deep-penetrating NIR-II QDs can be used for the tracking of ICH and BBB hyperpermeability in transgenic mice models of cerebral vascular anomalies.