Open AccessViscoelasticity of hydrazone crosslinked poly(ethylene glycol) hydrogels directs chondrocyte morphology during mechanical deformation
Author(s) -
Benjamin M. Richardson,
Cierra J. Walker,
Laura J. Macdougall,
Jack W Hoye,
Mark A. Randolph,
Stephanie J. Bryant,
Kristi S. Anseth
Publication year - 2020
Publication title -
biomaterials science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.422
H-Index - 64
eISSN - 2047-4849
pISSN - 2047-4830
DOI - 10.1039/d0bm00860e
Subject(s) - self healing hydrogels , viscoelasticity , hydrazone , ethylene glycol , chondrocyte , chemistry , biophysics , morphology (biology) , covalent bond , materials science , polymer chemistry , composite material , biochemistry , stereochemistry , organic chemistry , biology , in vitro , genetics
Chondrocyte deformation influences disease progression and tissue regeneration in load-bearing joints. In this work, we found that viscoelasticity of dynamic covalent crosslinks temporally modulates the biophysical transmission of physiologically relevant compressive strains to encapsulated chondrocytes. Chondrocytes in viscoelastic alky-hydrazone hydrogels demonstrated (91.4 ± 4.5%) recovery of native rounded morphologies during mechanical deformation, whereas primarily elastic benzyl-hydrazone hydrogels significantly limited morphological recovery (21.2 ± 1.4%).