Open AccessAn integrated self-powered 3D printed sample concentrator for highly sensitive molecular detection of HIV in whole blood at the point of care
Author(s) -
Karteek Kadimisetty,
Kun Yin,
Aoife M. Roche,
Yanjie Yi,
Frederic D. Bushman,
Ronald G. Collman,
Robert Gross,
Liang Feng,
Changchun Liu
Publication year - 2021
Publication title -
analyst (london. 1877. online)/analyst
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 153
eISSN - 1364-5528
pISSN - 0003-2654
DOI - 10.1039/d0an02482a
Subject(s) - concentrator , point of care , nucleic acid , sample preparation , lysis , human immunodeficiency virus (hiv) , sample (material) , point of care testing , chromatography , chemistry , materials science , virology , computer science , immunology , medicine , biochemistry , pathology , telecommunications
Rapid and efficient biological sample preparation and pretreatment are crucial for highly sensitive, reliable and reproducible molecular detection of infectious diseases. Herein, we report a self-powered, integrated sample concentrator (SPISC) for rapid plasma separation, pathogen lysis, nucleic acid trapping and enrichment at the point of care. The proposed sample concentrator uses a combination of gravitational sedimentation of blood cells and capillary force for rapid, self-powered plasma separation. The pathogens (e.g., HIV virus) in separated plasma were directly lysed and pathogen nucleic acid was enriched by an integrated, flow-through FTA® membrane in the concentrator, enabling highly efficient nucleic acid preparation. The FTA® membrane of the SPISC is easy to store and transport at room temperature without need for uninterrupted cold chain, which is crucial for point of care sampling in resource-limited settings. The platform has been successfully applied to detect HIV virus in blood samples. Our experiments show that the sample concentrator can achieve a plasma separation efficiency as high as 95% and a detection sensitivity as low as 10 copies per 200 μL blood (∼100 copies per mL plasma) with variability less than 7%. The sample concentrator described is fully compatible with downstream nucleic acid detection and has great potential for early diagnostics, monitoring and management of infectious diseases at the point of care.