Open AccessScalable syntheses of traceable ribosylated NAD+ precursors
Author(s) -
Mikhail V. Makarov,
Nekoda W. Harris,
Marcelo Rodrigues Rodrigues,
Marie E. Migaud
Publication year - 2019
Publication title -
organic and biomolecular chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 146
eISSN - 1477-0539
pISSN - 1477-0520
DOI - 10.1039/c9ob01981b
Subject(s) - nad+ kinase , chemistry , scalability , stereochemistry , nanotechnology , combinatorial chemistry , enzyme , computer science , biochemistry , database , materials science
Nicotinamide adenine dinucleotide, NAD + , is an essential cofactor and substrate for many cellular enzymes. Its sustained intracellular levels have been linked to improved physiological end points in a range of metabolic diseases. Biosynthetic precursors to NAD + include nicotinic acid, nicotinamide, the ribosylated parents and the phosphorylated form of the ribosylated parents. By combining solvent-assisted mechanochemistry and sealed reaction conditions, access to the ribosylated NAD + precursors and to the isotopologues of NAD + precursors was achieved in high yields and levels of purity. The latter is critical as it offers means to better trace biosynthetic pathways to NAD + , investigate the multifaceted roles of the intracellular NAD + pools, and better exploit NAD + biology.