Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML | Zendy

Jinzhu Yu | Zendy; Ki Oh | Zendy; Sitapriya Moorthi | Zendy; Ling Li | Zendy; Helmut H. Strey | Zendy; Michael Schuster | Zendy; Chiara Luberto | Zendy; PhenixLan Quan | Zendy; Eric Brouzés | Zendy

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Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

Author(s) -

Jinzhu Yu,

Ki Oh,

Sitapriya Moorthi,

Ling Li,

Helmut H. Strey,

Michael Schuster,

Chiara Luberto,

PhenixLan Quan,

Eric Brouzés

Publication year - 2020

Publication title -

lab on a chip

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.064

H-Index - 210

eISSN - 1473-0197

pISSN - 1473-0189

DOI - 10.1039/c9lc01213c

Subject(s) - flow cytometry , cytometry , enzyme , cell , breakpoint cluster region , mass cytometry , chemistry , abl , microbiology and biotechnology , cancer research , biology , biochemistry , tyrosine kinase , signal transduction , receptor , gene , phenotype

We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.

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