Open AccessEnzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML
Author(s) -
Yu Jia,
Ki Oh,
Sitapriya Moorthi,
Ling Li,
Helmut H. Strey,
Michael W. Schuster,
Chiara Luberto,
PhenixLan Quan,
Eric Brouzés
Publication year - 2020
Publication title -
lab on a chip
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.064
H-Index - 210
eISSN - 1473-0197
pISSN - 1473-0189
DOI - 10.1039/c9lc01213c
Subject(s) - flow cytometry , cytometry , intracellular , cell , enzyme , population , mass cytometry , breakpoint cluster region , single cell analysis , chemistry , microbiology and biotechnology , biology , biochemistry , medicine , receptor , environmental health , gene , phenotype
We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.