Open AccessA cephalosporin–chemiluminescent conjugate increases beta-lactamase detection sensitivity by four orders of magnitude
Author(s) -
Santanu Maity,
Xiaojian Wang,
Das S,
Maomao He,
Lee W. Riley,
Niren Murthy
Publication year - 2020
Publication title -
chemical communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.837
H-Index - 333
eISSN - 1364-548X
pISSN - 1359-7345
DOI - 10.1039/c9cc09498a
Subject(s) - chemiluminescence , cephalosporin , conjugate , chemistry , beta (programming language) , beta lactamase , substrate (aquarium) , sensitivity (control systems) , chromatography , antibiotics , biology , biochemistry , mathematics , computer science , escherichia coli , mathematical analysis , ecology , gene , programming language , electronic engineering , engineering
The expression of beta-lactamases in bacteria is a central cause of drug resistance. In this report, we present a beta-lactamase chemiluminescent probe, termed CCP, which can for the first time detect beta-lactamase activity via chemiluminescence and can detect beta lactamase with a sensitivity that is 4-orders of magnitude higher than the commercially available fluorescent lactamase substrate fluorocillin.