Open AccessThe methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols
Author(s) -
Jeremy David Adams,
Joachim Justad Røise,
David Sam Lee,
Niren Murthy
Publication year - 2020
Publication title -
chemical communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.837
H-Index - 333
eISSN - 1364-548X
pISSN - 1359-7345
DOI - 10.1039/c9cc09136j
Subject(s) - autocatalysis , chemistry , chain reaction , autocatalytic reaction , enzyme , combinatorial chemistry , chain (unit) , biochemistry , photochemistry , catalysis , physics , statistical physics , astronomy
We present an autocatalytic system for the detection and amplification of thiols termed the Methionase Chain Reaction (MCR). MCR is based on the reversible modification of the thiol producing enzyme Methionine Gamma-Lyase (MGL). MCR was able to amplify the concentration of thiols by a factor of 560 and was able to visually detect thiols at concentrations as low as 50 nM.