Open AccessA ‘light-up’ intercalator displacement assay for detection of triplex DNA stabilizers
Author(s) -
Imee Marie A. del Mundo,
Eun Jeong Cho,
Kevin N. Dalby,
Karen M. Vásquez
Publication year - 2020
Publication title -
chemical communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.837
H-Index - 333
eISSN - 1364-548X
pISSN - 1359-7345
DOI - 10.1039/c9cc08817b
Subject(s) - intercalation (chemistry) , dna , chemistry , displacement (psychology) , combinatorial chemistry , high throughput screening , throughput , biophysics , chromatography , biochemistry , organic chemistry , biology , computer science , psychology , psychotherapist , telecommunications , wireless
Here, we developed a coralyne-based, 'light-up' intercalator displacement assay to identify molecular stabilizers of triplex DNA using a sequence from a chromosomal breakpoint hotspot in the human c-MYC oncogene. Its potential to identify triplex DNA ligands was demonstrated using BePI and doxorubicin. Identification of triplex-interacting ligands may allow the regulation of genetic instability in human genomes.