Open AccessEnzymatic reaction-based nanopore detection of zinc ions
Author(s) -
Golbarg M Roozbahani,
Youwen Zhang,
Xiaohan Chen,
Mona Hoseini Soflaee,
Xiyun Guan
Publication year - 2019
Publication title -
analyst (london. 1877. online)/analyst
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 153
eISSN - 1364-5528
pISSN - 0003-2654
DOI - 10.1039/c9an01784d
Subject(s) - nanopore , chemistry , zinc , enzyme , ion , combinatorial chemistry , biochemistry , nanotechnology , materials science , organic chemistry
We report a label-free nanopore sensor for the detection of Zn2+ ions. By taking advantage of the cleavage of a substrate peptide by zinc-dependent enzymes, nanomolar concentrations of Zn2+ ions could be detected within minutes. Furthermore, structurally similar transition metals such as Ni2+, Co2+, Hg2+, Cu2+, and Cd2+ did not interfere with their detection. The enzymatic reaction-based nanopore sensing strategy developed in this work may find potential applications in environmental monitoring and medical diagnosis.