Open Access64Cu-Labeled multifunctional dendrimers for targeted tumor PET imaging
Author(s) -
Wenhui Ma,
Fanfan Fu,
Jingyi Zhu,
Rui Huang,
YiZhou Zhu,
Zhenwei Liu,
Jing Wang,
Peter S. Conti,
Xiangyang Shi,
Kai Chen
Publication year - 2018
Publication title -
nanoscale
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.038
H-Index - 224
eISSN - 2040-3372
pISSN - 2040-3364
DOI - 10.1039/c7nr09269e
Subject(s) - dendrimer , folate receptor , pet imaging , folic acid , molecular imaging , chemistry , radiochemistry , positron emission tomography , materials science , cancer , nuclear medicine , cancer cell , biochemistry , medicine , in vivo , biology , microbiology and biotechnology
We report the use of multifunctional folic acid (FA)-modified dendrimers as a platform to radiolabel with 64Cu for PET imaging of folate receptor (FR)-expressing tumors. In this study, amine-terminated generation 5 (G5) poly(amidoamine) dendrimers were sequentially modified with fluorescein isothiocyanate (FI), FA, and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), followed by acetylation of the remaining dendrimer terminal amines. The as-formed multifunctional DOTA-FA-FI-G5·NHAc dendrimers were then radiolabeled with 64Cu via the DOTA chelation. We show that the FA modification renders the dendrimers with targeting specificity to cancer cells overexpressing FR in vitro. Importantly, the radiolabeled 64Cu-DOTA-FA-FI-G5·NHAc dendrimers can be used as a nanoprobe for specific targeting of FR-overexpressing cancer cells in vitro and targeted microPET imaging of the FR-expressing xenografted tumor model in vivo. The developed 64Cu-labeled multifunctional dendrimeric nanoprobe may hold great promise to be used for targeted PET imaging of different types of FR-expressing cancer.