RNA triplex-to-duplex and duplex-to-triplex conversion induced by coralyne | Zendy

Francisco J. Hoyuelos | Zendy; Begoña Garcı́a | Zendy; José M. Leal | Zendy; Natalia Busto | Zendy; Tarita Biver | Zendy; Fernando Secco | Zendy; Marcella Venturini | Zendy

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RNA triplex-to-duplex and duplex-to-triplex conversion induced by coralyne

Author(s) -

Francisco J. Hoyuelos,

Begoña Garcı́a,

José M. Leal,

Natalia Busto,

Tarita Biver,

Fernando Secco,

Marcella Venturini

Publication year - 2014

Publication title -

physical chemistry chemical physics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.053

H-Index - 239

eISSN - 1463-9084

pISSN - 1463-9076

DOI - 10.1039/c3cp52270a

Subject(s) - duplex (building) , circular dichroism , chemistry , fluorescence , rna , crystallography , biophysics , stereochemistry , dna , biochemistry , biology , optics , physics , gene

Spectrophotometric, circular dichroism, calorimetric, displacement assay and kinetic analyses of the binding of the fluorescent dye coralyne to poly(A)2poly(U) have served to enlighten the ability of the dye to produce dramatic changes in the RNA structure. The sets of data assembled convey that coralyne is able to induce the triplex-to-duplex conversion and also the duplex-to-triplex conversion according to a non-reversible cycle governed by temperature, provided that the [dye]/[polymer] ratio (CD/CP) is maintained constant above unity. Alternatively, at room temperature the triplex is formed at (roughly) CD/CP < 1 and the duplex at CD/CP > 1.

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