Identification and isolation of lantibiotics from culture: a bioorthogonal chemistry approach | Zendy

Jing Li | Zendy; Geneviève Girard | Zendy; Bogdan I. Florea | Zendy; Paul P. Geurink | Zendy; Nan Li | Zendy; Gijsbert A. van der Marel | Zendy; Mark Overhand | Zendy; Herman S. Overkleeft | Zendy; Gilles P. van Wezel | Zendy

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Identification and isolation of lantibiotics from culture: a bioorthogonal chemistry approach

Author(s) -

Jing Li,

Geneviève Girard,

Bogdan I. Florea,

Paul P. Geurink,

Nan Li,

Gijsbert A. van der Marel,

Mark Overhand,

Herman S. Overkleeft,

Gilles P. van Wezel

Publication year - 2012

Publication title -

organic and biomolecular chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 146

eISSN - 1477-0539

pISSN - 1477-0520

DOI - 10.1039/c2ob26050f

Subject(s) - lantibiotics , chemistry , bioorthogonal chemistry , residue (chemistry) , nisin , isolation (microbiology) , amino acid residue , biochemistry , combinatorial chemistry , amino acid , stereochemistry , peptide sequence , organic chemistry , microbiology and biotechnology , click chemistry , antimicrobial , biology , gene

A distinguishing feature of the lantibiotic family of cyclic peptides is the presence of thioethers. Treatment of a lantibiotic with an alkaline solution at high pH gives rise to a β-elimination reaction yielding the corresponding ring opened precursor, containing a dehydro-amino acid residue. We here reveal in a proof-of-concept study that a ring opened lantibiotic (mersacidin) can be captured for pull-down from a culture broth, subsequently released and identified by mass spectrometry.

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