Gangliosides' analysis by MALDI-ion mobility MS | Zendy

Shelley N. Jackson | Zendy; Benoît Colsch | Zendy; Thomas F. Egan | Zendy; Ernest K. Lewis | Zendy; J. Schultz | Zendy; Amina S. Woods | Zendy

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Gangliosides' analysis by MALDI-ion mobility MS

Author(s) -

Shelley N. Jackson,

Benoît Colsch,

Thomas F. Egan,

Ernest K. Lewis,

J. Schultz,

Amina S. Woods

Publication year - 2010

Publication title -

the analyst

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.998

H-Index - 153

eISSN - 1364-5528

pISSN - 0003-2654

DOI - 10.1039/c0an00732c

Subject(s) - chemistry , biomolecule , sialic acid , matrix assisted laser desorption/ionization , ion , ganglioside , ion mobility spectrometry , isobaric process , desorption , chromatography , mass spectrometry , peptide , biochemistry , organic chemistry , physics , adsorption , thermodynamics

The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis of biomolecules in complex mixtures (such as tissue sections and cellular extracts), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class of complex glycosphingolipids that has different degrees of sialylation. Both GD1a and GD1b, structural isomers, were studied to see the effects on gas-phase structure depending upon the localization of the sialic acids. A total ganglioside extract from mouse brain was also analyzed to measure the effectiveness of ion mobility to separate out the different ganglioside species in a complex mixture.

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