Open AccessGangliosides' analysis by MALDI-ion mobility MS
Author(s) -
Shelley N. Jackson,
Benoît Colsch,
Thomas F. Egan,
Ernest K. Lewis,
J. Schultz,
Amina S. Woods
Publication year - 2011
Publication title -
analyst (london. 1877. online)/analyst
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 153
eISSN - 1364-5528
pISSN - 0003-2654
DOI - 10.1039/c0an00732c
Subject(s) - chemistry , biomolecule , sialic acid , matrix assisted laser desorption/ionization , ion , ganglioside , ion mobility spectrometry , isobaric process , desorption , chromatography , mass spectrometry , peptide , biochemistry , organic chemistry , physics , adsorption , thermodynamics
The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis of biomolecules in complex mixtures (such as tissue sections and cellular extracts), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class of complex glycosphingolipids that has different degrees of sialylation. Both GD1a and GD1b, structural isomers, were studied to see the effects on gas-phase structure depending upon the localization of the sialic acids. A total ganglioside extract from mouse brain was also analyzed to measure the effectiveness of ion mobility to separate out the different ganglioside species in a complex mixture.