Open AccessInteraction and oxidative damage of DVDMS to BSA: a study on the mechanism of photodynamic therapy-induced cell death
Author(s) -
Li Li,
Huiyu Wang,
Haiping Wang,
Lijun Li,
Pan Wang,
Xiaobing Wang,
Quanhong Liu
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep43324
Subject(s) - photodynamic therapy , reactive oxygen species , cytotoxicity , apoptosis , bovine serum albumin , programmed cell death , chemistry , photosensitizer , viability assay , oxidative damage , cell , cancer research , oxidative stress , biochemistry , photochemistry , in vitro , medicine , organic chemistry
Photodynamic therapy (PDT) is a promising method for neoplastic and nonneoplastic diseases. In this study, we utilized sinoporphyrin sodium (DVDMS) as a sensitizer combined with light to investigate its cytotoxic effect on different cell lines. For this purpose, we chose bovine serum albumin (BSA) as a model to explore the mechanism of PDT-induced cell death at a molecular level. Our findings indicated that the combined treatment significantly suppressed cell survival. Fluorescence spectroscopy revealed a strong interaction between DVDMS and BSA molecules in aqueous solution, affecting DVDMS’ targeting distribution and metabolism. Spectroscopic analysis and carbonyl content detection indicated that DVDMS-PDT significantly enhanced the damage of BSA at a higher extent than Photofrin II-PDT under similar experimental conditions. Our observations were consistent with the cytotoxicity results. Excessive reactive oxygen species (ROS) were induced by the synergy effect of the sensitizer and light, which played an important role in damaging BSA and tumor cells. These results suggested that the interaction and oxidative damage of protein molecules by DVDMS were the main reasons to cell death and constitute a valuable reference for future DVDMS-PDT investigations.