Open AccessDynamic changes in the transcriptome of Populus hopeiensis in response to abscisic acid
Author(s) -
Zhong Chen,
Lexiang Ji,
Jia Wang,
Jinpu Jin,
Xiaoyu Yang,
Pian Rao,
Kai Gao,
Weihua Liao,
Meixia Ye,
Xinmin An
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep42708
Subject(s) - abscisic acid , wrky protein domain , transcriptome , myb , biology , abiotic stress , gene expression , gene ontology , drought tolerance , transcription factor , gene , abiotic component , botany , gene expression profiling , adaptation (eye) , metabolomics , computational biology , microbiology and biotechnology , genetics , bioinformatics , ecology , neuroscience
Abscisic acid (ABA) plays a fundamental role in plant response and adaptation to abiotic stresses, such as drought, high salinity and low temperature. Populus hopeiensis exhibits exceptional tolerance to water-deficit environments and is therefore an excellent choice for studying drought tolerance in trees. This study provides a global view of transcriptome dynamics in P. hopeiensis in response to exogenous ABA using Illumina RNA-sequencing. Endogenous ABA content increased and reached a peak at 8 h after ABA treatment and then significantly decreased at latter time points. Differential expression analysis and Gene ontology enrichment revealed that the number of transcripts exhibited significant increase during the first 8 hours after ABA treatment, which then significantly decreased at 12 and 24 h. Transcription factors (TFs) analysis showed that six different patterns were observed based on the expression of the six TFs families (AP2/ERF, NAC, MYB, MYB-related, bZIP and WRKY) and the majority of differentially expressed TFs increased rapidly after ABA treatment. This study provides a robust resource for investigating the functions of genes induced by ABA and will help to develop a better understanding of the molecular regulatory mechanism in response to drought in poplar.