Open AccessMulti-omics analyses reveal metabolic alterations regulated by hepatitis B virus core protein in hepatocellular carcinoma cells
Author(s) -
Qiang Xie,
Fengxu Fan,
Wei Wei,
Liu Yang,
Zhongwei Xu,
Linhui Zhai,
Yingzi Qi,
Bingyu Ye,
Yao Zhang,
Sumit Basu,
Zhihu Zhao,
Junzhu Wu,
Ping Xu
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep41089
Subject(s) - hepatocellular carcinoma , hepatitis b virus , proteomics , transfection , hbx , glycolysis , hepatitis c virus , pathogenesis , virus , viral life cycle , biology , cancer research , virology , enzyme , viral replication , gene , immunology , biochemistry
Chronic hepatitis B virus (HBV) infection is partly responsible for hepatitis, fatty liver disease and hepatocellular carcinoma (HCC). HBV core protein (HBc), encoded by the HBV genome, may play a significant role in HBV life cycle. However, the function of HBc in the occurrence and development of liver disease is still unclear. To investigate the underlying mechanisms, HBc-transfected HCC cells were characterized by multi-omics analyses. Combining proteomics and metabolomics analyses, our results showed that HBc promoted the expression of metabolic enzymes and the secretion of metabolites in HCC cells. In addition, glycolysis and amino acid metabolism were significantly up-regulated by HBc. Moreover, Max-like protein X (MLX) might be recruited and enriched by HBc in the nucleus to regulate glycolysis pathways. This study provides further insights into the function of HBc in the molecular pathogenesis of HBV-induced diseases and indicates that metabolic reprogramming appears to be a hallmark of HBc transfection.