Open AccessCrystal structure of Sec10, a subunit of the exocyst complex
Author(s) -
Jianxing Chen,
Atsushi Yamagata,
Keiko Kubota,
Yusuke Sato,
Sakurako Goto-Ito,
Shuya Fukai
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep40909
Subject(s) - exocyst , antiparallel (mathematics) , protein subunit , gtpase , crystallography , microbiology and biotechnology , biology , vesicular transport proteins , chemistry , biophysics , endosome , biochemistry , physics , quantum mechanics , magnetic field , gene , vacuolar protein sorting , intracellular
The exocyst complex is a heterooctameric protein complex composed of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84. This complex plays an essential role in trafficking secretory vesicles to the plasma membrane through its interaction with phosphatidylinositol 4,5-bisphosphate and small GTPases. To date, the near-full-length structural information of each subunit has been limited to Exo70, although the C-terminal half structures of Sec6, Sec15 and Exo84 and the structures of the small GTPase-binding domains of Sec3, Sec5 and Exo84 have been reported. Here, we report the crystal structure of the near-full-length zebrafish Sec10 (zSec10) at 2.73 Å resolution. The structure of zSec10 consists of tandem antiparallel helix bundles that form a straight rod, like helical core regions of other exocyst subunits. This structure provides the first atomic details of Sec10, which may be useful for future functional and structural studies of this subunit and the exocyst complex.