Open AccessDopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125
Author(s) -
Junkai Gao,
Yanjun Jiang,
Jinshu Lu,
Zhi Han,
Jiajia Deng,
Yan Chen
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep40395
Subject(s) - lipase , dopamine , candida antarctica , mesoporous silica , matrix (chemical analysis) , mesoporous material , chemistry , chromatography , enzyme , biochemistry , biology , neuroscience , catalysis
Dopmine functionalized mesoporous onion-like silica (DPMS) was synthesized via a biomimetic coating, and lipase Candida sp. 99-125 (LCS) was immobilized in DPMS (LCS@DPMS) by physical adsorption in this study. The DPMS was characterized by SEM, TEM, BET and FT-IR, and it was shown that the DPMS had clear multishell structures with large surface area of 419 m 2 /g. The activity, pH stability, thermal stability, storage stability, and reusability of the LCS@DPMS were investigated in detail. The stabilities of LCS@DPMS were improved significantly compared to the free lipase and LCS@MS (LCS immobilized in unfunctionalized mesoporous onion-like silica by physical adsorption). All the results indicated that the DPMS had high efficiency and improved stability for lipase immobilization.