Open AccessIsolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting
Author(s) -
Georgia K. Atkin-Smith,
Stephanie Paone,
Damien Zanker,
Mubing Duan,
Than K. Phan,
Weisan Chen,
Mark D. Hulett,
Ivan K. H. Poon
Publication year - 2017
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep39846
Subject(s) - cell sorting , microbiology and biotechnology , cell , extracellular , apoptosis , cell type , intracellular , isolation (microbiology) , extracellular vesicles , sorting , cell culture , cell membrane , chemistry , biology , biochemistry , bioinformatics , computer science , genetics , programming language
Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.