Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting | Zendy

Georgia K. AtkinSmith | Zendy; Stephanie Paone | Zendy; Damien Zanker | Zendy; Mubing Duan | Zendy; Thanh Kha Phan | Zendy; Weisan Chen | Zendy; Mark D. Hulett | Zendy; Ivan K. H. Poon | Zendy

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Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting

Author(s) -

Georgia K. AtkinSmith,

Stephanie Paone,

Damien Zanker,

Mubing Duan,

Thanh Kha Phan,

Weisan Chen,

Mark D. Hulett,

Ivan K. H. Poon

Publication year - 2017

Publication title -

scientific reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.24

H-Index - 213

ISSN - 2045-2322

DOI - 10.1038/srep39846

Subject(s) - cell sorting , microbiology and biotechnology , cell , extracellular , apoptosis , cell type , intracellular , isolation (microbiology) , extracellular vesicles , sorting , cell culture , cell membrane , chemistry , biology , biochemistry , bioinformatics , computer science , genetics , programming language

Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.

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