
Multimodal Sensing Strategy Using pH Dependent Fluorescence Switchable System
Author(s) -
Alagan Muthurasu,
V. Ganesh
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep39452
Subject(s) - fluorescence , computer science , chemistry , biophysics , biology , physics , quantum mechanics
Biomolecules assisted preparation of fluorescent gold nanoparticles (FL–Au NPs) has been reported in this work using glucose oxidase enzyme as both reducing and stabilizing agent and demonstrated their application through multimodal sensing strategy for selective detection of cysteine (Cys). Three different methods namely fluorescence turn OFF–ON strategy, naked eye detection and electrochemical methods are used for Cys detection by employing FL–Au NPs as a common probe. In case of fluorescence turn–OFF method a strong interaction between Au NPs and thiol results in quenching of fluorescence due to replacement of glucose oxidase by Cys at neutral pH. Second mode is based on fluorescence switch–ON strategy where initial fluorescence is significantly quenched by either excess acid or base and further addition of Cys results in appearance of rosy-red and green fluorescence respectively. Visual colour change and fluorescence emission arises due to etching of Au atoms on the surface by thiol leading to formation of Au nanoclusters. Finally, electrochemical sensing of Cys is also carried out using cyclic voltammetry in 0.1 M PBS solution. These findings provide a suitable platform for Cys detection over a wide range of pH and concentration levels and hence the sensitivity can also be tuned accordingly.