Open AccessThe presence of T cell epitopes is important for induction of antibody responses against antigens directed to DEC205+ dendritic cells
Author(s) -
Kelly N. S. Amorim,
Eline Vital Rampazo,
Renan Antonialli,
Márcio Yamamoto,
Maurício M. Rodrigues,
Irene da Silva Soares,
Sílvia Beatriz Boscardin
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep39250
Subject(s) - epitope , antigen , dendritic cell , antibody , antibody response , biology , immunology , antigen presenting cell , t cell , microbiology and biotechnology , computational biology , immune system
In vivo antigen targeting to dendritic cells (DCs) has been used as a way to improve immune responses. Targeting is accomplished with the use of monoclonal antibodies (mAbs) to receptors present on the DC surface fused with the antigen of interest. An anti-DEC205 mAb has been successfully used to target antigens to the DEC205 + CD8α + DC subset. The administration of low doses of the hybrid mAb together with DC maturation stimuli is able to activate specific T cells and induce production of high antibody titres for a number of different antigens. However, it is still not known if this approach would work with any fused protein. Here we genetically fused the αDEC205 mAb with two fragments (42-kDa and 19-kDa) derived from the ~200 kDa Plasmodium vivax merozoite surface protein 1 (MSP1), known as MSP1 42 and MSP1 19 , respectively. The administration of two doses of αDEC-MSP1 42 , but not of αDEC-MSP1 19 mAb, together with an adjuvant to two mouse strains induced high anti-MSP1 19 antibody titres that were dependent on CD4 + T cells elicited by peptides present in the MSP1 33 sequence, indicating that the presence of T cell epitopes in antigens targeted to DEC205 + DCs increases antibody responses.