Open AccessNaa50/San-dependent N-terminal acetylation of Scc1 is potentially important for sister chromatid cohesion
Author(s) -
Ana Ribeiro,
Rui D. Silva,
Håvard Foyn,
Margarida N. Tiago,
Om Singh Rathore,
Thomas Arnesen,
Rui Gonçalo Martinho
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep39118
Subject(s) - establishment of sister chromatid cohesion , sister chromatids , terminal (telecommunication) , sister , genetics , cohesion (chemistry) , computer science , acetylation , biology , chemistry , chromosome , computer network , gene , political science , organic chemistry , law
The gene separation anxiety (san ) encodes Naa50/San, a N-terminal acetyltransferase required for chromosome segregation during mitosis. Although highly conserved among higher eukaryotes, the mitotic function of this enzyme is still poorly understood. Naa50/San was originally proposed to be required for centromeric sister chromatid cohesion in Drosophila and human cells, yet, more recently, it was also suggested to be a negative regulator of microtubule polymerization through internal acetylation of beta Tubulin. We used genetic and biochemical approaches to clarify the function of Naa50/San during development. Our work suggests that Naa50/San is required during tissue proliferation for the correct interaction between the cohesin subunits Scc1 and Smc3. Our results also suggest a working model where Naa50/San N-terminally acetylates the nascent Scc1 polypeptide, and that this co-translational modification is subsequently required for the establishment and/or maintenance of sister chromatid cohesion.