Progesterone Receptor Membrane Component 1 Mediates Progesterone-Induced Suppression of Oocyte Meiotic Prophase I and Primordial Folliculogenesis | Zendy

Meng Guo | Zendy; Cheng Zhang | Zendy; Yan Wang | Zendy; Lizhao Feng | Zendy; Zhengpin Wang | Zendy; Wanbo Niu | Zendy; Xiaoyan Du | Zendy; Wang Tang | Zendy; Yuna Li | Zendy; Chao Wang | Zendy; Zhenwen Chen | Zendy

Open Access

Progesterone Receptor Membrane Component 1 Mediates Progesterone-Induced Suppression of Oocyte Meiotic Prophase I and Primordial Folliculogenesis

Author(s) -

Meng Guo,

Cheng Zhang,

Yan Wang,

Lizhao Feng,

Zhengpin Wang,

Wanbo Niu,

Xiaoyan Du,

Wang Tang,

Yuna Li,

Chao Wang,

Zhenwen Chen

Publication year - 2016

Publication title -

scientific reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.24

H-Index - 213

ISSN - 2045-2322

DOI - 10.1038/srep36869

Subject(s) - folliculogenesis , oocyte , progesterone receptor , meiosis , microbiology and biotechnology , prophase , oogenesis , chemistry , component (thermodynamics) , medicine , biology , endocrinology , genetics , physics , gene , embryogenesis , estrogen receptor , embryo , cancer , breast cancer , thermodynamics

Well-timed progression of primordial folliculogenesis is essential for mammalian female fertility. Progesterone (P4) inhibits primordial follicle formation under physiological conditions; however, P4 receptor that mediates this effect and its underlying mechanisms are unclear. In this study, we used an in vitro organ culture system to show that progesterone receptor membrane component 1 (PGRMC1) mediated P4-induced inhibition of oocyte meiotic prophase I and primordial follicle formation. We found that membrane-impermeable BSA-conjugated P4 inhibited primordial follicle formation similar to that by P4. Interestingly, PGRMC1 and its partner serpine1 mRNA-binding protein 1 were highly expressed in oocytes in perinatal ovaries. Inhibition or RNA interference of PGRMC1 abolished the suppressive effect of P4 on follicle formation. Furthermore, P4-PGRMC1 interaction blocked oocyte meiotic progression and decreased intra-oocyte cyclic AMP (cAMP) levels in perinatal ovaries. cAMP analog dibutyryl cAMP reversed P4–PGRMC1 interaction-induced inhibition of meiotic progression and follicle formation. Thus, our results indicated that PGRMC1 mediated P4-induced suppression of oocyte meiotic progression and primordial folliculogenesis by decreasing intra-oocyte cAMP levels.

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