z-logo
open-access-imgOpen Access
NLRP3 inflammasome mediates contrast media-induced acute kidney injury by regulating cell apoptosis
Author(s) -
Jianxiao Shen,
Ling Wang,
Na Jiang,
Shan Mou,
Minfang Zhang,
Leyi Gu,
Xinghua Shao,
Qin Wang,
Qi Chen,
Shu Li,
Wanpeng Wang,
Xiajing Che,
Zhaohui Ni
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep34682
Subject(s) - inflammasome , apoptosis , proinflammatory cytokine , acute kidney injury , inflammation , medicine , cancer research , microbiology and biotechnology , pyroptosis , immunology , biology , biochemistry
Iodinated contrast media serves as a direct causative factor of acute kidney injury (AKI) and is involved in the progression of cellular dysfunction and apoptosis. Emerging evidence indicates that NLRP3 inflammasome triggers inflammation, apoptosis and tissue injury during AKI. Nevertheless, the underlying renoprotection mechanism of NLRP3 inflammasome against contrast-induced AKI (CI-AKI) was still uncertain. This study investigated the role of NLRP3 inflammasome in CI-AKI both in vitro and in vivo . In HK-2 cells and unilateral nephrectomy model, NLRP3 and NLRP3 inflammasome member ASC were significantly augmented with the treatment of contrast media. Moreover, genetic disruption of NLRP3 notably reversed contrast-induced expression of apoptosis related proteins and secretion of proinflammatory factors, similarly to the effects of ASC deletion. Consistent with above results, absence of NLRP3 in mice undergoing unilateral nephrectomy also protected against contrast media-induced renal cells phenotypic alteration and cell apoptosis via modulating expression level of apoptotic proteins. Collectively, we demonstrated that NLRP3 inflammasome mediated CI-AKI through modulating the apoptotic pathway, which provided a potential therapeutic target for the treatment of contrast media induced acute kidney injury.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here