Open AccessBiotransformation of ferulic acid to vanillin in the packed bed-stirred fermentors
Author(s) -
Lei Yan,
Peng Chen,
Shuang Zhang,
Suyue Li,
Xin Yan,
Ning Bo Wang,
Ning Liang,
Hongyu Li
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep34644
Subject(s) - vanillin , biotransformation , ferulic acid , chemistry , chromatography , bacillus subtilis , bioreactor , yield (engineering) , nuclear chemistry , biochemistry , organic chemistry , materials science , biology , bacteria , enzyme , genetics , metallurgy
We performed the biotransformation of ferulic acid to vanillin using Bacillus subtilis ( B. subtilis ) in the stirring packed-bed reactors filled with carbon fiber textiles (CFT). Scanning electron microscope (SEM), HPLC, qRT-PCR and ATP assay indicated that vanillin biotransformation is tightly related to cell growth, cellar activity and the extent of biofilm formation. The biotransformation was affected by hydraulic retention time (HRT), temperature, initial pH, stirring speed and ferulic acid concentration, and the maximum vanillin production was obtained at 20 h, 35 °C, 9.0, 200 rpm, 1.5 g/L, respectively. Repeated batch biotransformation performed under this optimized condition showed that the maximum productivity (0.047 g/L/h) and molar yield (60.43%) achieved in immobilized cell system were 1.84 and 3.61 folds higher than those achieved in free cell system. Therefore, the stirring reactor packed with CFT carrier biofilm formed by B. subtilis represented a valid biocatalytic system for the production of vanillin.