Open AccessIdentification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans
Author(s) -
Erna Li,
Zhe Yin,
Yanyan Ma,
Huan Li,
Weishi Lin,
Xiao Wei,
Rong Zhao,
Aimin Jiang,
Jing Yuan,
Xiangna Zhao
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep34300
Subject(s) - achromobacter xylosoxidans , siphoviridae , bacteriophage , microbiology and biotechnology , lytic cycle , biology , myoviridae , bacteria , chemistry , gene , genetics , virus , escherichia coli
A novel Achromobacter xylosoxidans bacteriophage, phiAxp-2, was isolated from hospital sewage in China. The phage was morphologically and microbiologically characterized, and its one-step growth curve, host range, genomic sequence, and receptor were determined. Its morphology showed that phiAxp-2 belongs to the family Siphoviridae . Microbiological characterization demonstrated that pH 7 is most suitable for phage phiAxp-2; its titer decreased when the temperature exceeded 50 °C; phiAxp-2 is sensitive to ethanol and isopropanol; and the presence of calcium and magnesium ions is necessary to accelerate cell lysis and improve the formation of phiAxp-2 plaques. Genomic sequencing and a bioinformatic analysis showed that phage phiAxp-2 is a novel bacteriophage, consisting of a circular, double-stranded 62,220-bp DNA molecule with a GC content of 60.11% that encodes 86 putative open reading frames (ORFs). The lipopolysaccharide of A. xylosoxidans is involved in the adsorption of phiAxp-2.