Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans | Zendy

Erna Li | Zendy; Zhe Yin | Zendy; Yanyan Ma | Zendy; Huan Li | Zendy; Weishi Lin | Zendy; Xiao Wei | Zendy; Ruixiang Zhao | Zendy; Aimin Jiang | Zendy; Jing Yuan | Zendy; Xiangna Zhao | Zendy

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Identification and molecular characterization of bacteriophage phiAxp-2 of Achromobacter xylosoxidans

Author(s) -

Erna Li,

Zhe Yin,

Yanyan Ma,

Huan Li,

Weishi Lin,

Xiao Wei,

Ruixiang Zhao,

Aimin Jiang,

Jing Yuan,

Xiangna Zhao

Publication year - 2016

Publication title -

scientific reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.24

H-Index - 213

ISSN - 2045-2322

DOI - 10.1038/srep34300

Subject(s) - achromobacter xylosoxidans , siphoviridae , bacteriophage , microbiology and biotechnology , lytic cycle , biology , myoviridae , bacteria , chemistry , gene , genetics , virus , escherichia coli

A novel Achromobacter xylosoxidans bacteriophage, phiAxp-2, was isolated from hospital sewage in China. The phage was morphologically and microbiologically characterized, and its one-step growth curve, host range, genomic sequence, and receptor were determined. Its morphology showed that phiAxp-2 belongs to the family Siphoviridae . Microbiological characterization demonstrated that pH 7 is most suitable for phage phiAxp-2; its titer decreased when the temperature exceeded 50 °C; phiAxp-2 is sensitive to ethanol and isopropanol; and the presence of calcium and magnesium ions is necessary to accelerate cell lysis and improve the formation of phiAxp-2 plaques. Genomic sequencing and a bioinformatic analysis showed that phage phiAxp-2 is a novel bacteriophage, consisting of a circular, double-stranded 62,220-bp DNA molecule with a GC content of 60.11% that encodes 86 putative open reading frames (ORFs). The lipopolysaccharide of A. xylosoxidans is involved in the adsorption of phiAxp-2.

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