Genetic characterization of two fully sequenced multi-drug resistant plasmids pP10164-2 and pP10164-3 from Leclercia adecarboxylata | Zendy

Fengjun Sun | Zendy; Dongsheng Zhou | Zendy; Qiang Sun | Zendy; Wenbo Luo | Zendy; Yigang Tong | Zendy; Defu Zhang | Zendy; Qian Wang | Zendy; Wei Feng | Zendy; Weijun Chen | Zendy; Yahan Fan | Zendy; Peiyuan Xia | Zendy

Open Access

Genetic characterization of two fully sequenced multi-drug resistant plasmids pP10164-2 and pP10164-3 from Leclercia adecarboxylata

Author(s) -

Fengjun Sun,

Dongsheng Zhou,

Qiang Sun,

Wenbo Luo,

Yigang Tong,

Defu Zhang,

Qian Wang,

Wei Feng,

Weijun Chen,

Yahan Fan,

Peiyuan Xia

Publication year - 2016

Publication title -

scientific reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.24

H-Index - 213

ISSN - 2045-2322

DOI - 10.1038/srep33982

Subject(s) - plasmid , mobile genetic elements , biology , insertion sequence , transposable element , microbiology and biotechnology , genetics , multiple drug resistance , drug resistance , gene , genome

We previously reported the complete sequence of the resistance plasmid pP10164-NDM, harboring bla NDM (conferring carbapenem resistance) and ble MBL (conferring bleomycin resistance), which is recovered from a clinical Leclercia adecarboxylata isolate P10164 from China. This follow-up work disclosed that there were still two multidrug-resistant (MDR) plasmids pP10164-2 and pP10164-3 coexisting in this strain. pP10164-2 and pP10164-3 were completely sequenced and shown to carry a wealth of resistance genes, which encoded the resistance to at least 10 classes of antibiotics (β-lactams. macrolides, quinolones, aminoglycosides, tetracyclines, amphenicols, quaternary ammonium compounds, sulphonamides, trimethoprim, and rifampicin) and 7 kinds of heavy mental (mercury, silver, copper, nickel, chromate, arsenic, and tellurium). All of these antibiotic resistance genes are associated with mobile elements such as transposons, integrons, and insertion sequence-based transposable units, constituting a total of three novel MDR regions, two in pP10164-2 and the other one in pP10164-3. Coexistence of three resistance plasmids pP10164-NDM, pP10164-2 and pP10164-3 makes L. adecarboxylata P10164 tend to become extensively drug-resistant.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research