Open AccessGenetic characterization of two fully sequenced multi-drug resistant plasmids pP10164-2 and pP10164-3 from Leclercia adecarboxylata
Author(s) -
Fengjun Sun,
Dongsheng Zhou,
Qiang Sun,
Wenbo Luo,
Yigang Tong,
Defu Zhang,
Qian Wang,
Wei Feng,
Weijun Chen,
Yuxia Fan,
Peiyuan Xia
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep33982
Subject(s) - plasmid , mobile genetic elements , biology , insertion sequence , transposable element , microbiology and biotechnology , genetics , multiple drug resistance , drug resistance , gene , genome
We previously reported the complete sequence of the resistance plasmid pP10164-NDM, harboring bla NDM (conferring carbapenem resistance) and ble MBL (conferring bleomycin resistance), which is recovered from a clinical Leclercia adecarboxylata isolate P10164 from China. This follow-up work disclosed that there were still two multidrug-resistant (MDR) plasmids pP10164-2 and pP10164-3 coexisting in this strain. pP10164-2 and pP10164-3 were completely sequenced and shown to carry a wealth of resistance genes, which encoded the resistance to at least 10 classes of antibiotics (β-lactams. macrolides, quinolones, aminoglycosides, tetracyclines, amphenicols, quaternary ammonium compounds, sulphonamides, trimethoprim, and rifampicin) and 7 kinds of heavy mental (mercury, silver, copper, nickel, chromate, arsenic, and tellurium). All of these antibiotic resistance genes are associated with mobile elements such as transposons, integrons, and insertion sequence-based transposable units, constituting a total of three novel MDR regions, two in pP10164-2 and the other one in pP10164-3. Coexistence of three resistance plasmids pP10164-NDM, pP10164-2 and pP10164-3 makes L. adecarboxylata P10164 tend to become extensively drug-resistant.