Open AccessPorcine milk-derived exosomes promote proliferation of intestinal epithelial cells
Author(s) -
Ting Chen,
Mei-Ying Xie,
Jiajie Sun,
Rui-Song Ye,
Xiao Cheng,
Rui-Ping Sun,
Limin Wei,
Meng Li,
Delin Lin,
Qingyan Jiang,
Qianyun Xi,
Yongliang Zhang
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep33862
Subject(s) - microvesicles , crypt , cell growth , flow cytometry , biology , western blot , microbiology and biotechnology , rna , exosome , proliferating cell nuclear antigen , intestinal mucosa , andrology , microrna , gene , medicine , endocrinology , biochemistry
Milk-derived exosomes were identified as a novel mechanism of mother-to-child transmission of regulatory molecules, but their functions in intestinal tissues of neonates are not well-studied. Here, we characterized potential roles of porcine milk-derived exosomes in the intestinal tract. In vitro , treatment with milk-derived exosomes (27 ± 3 ng and 55 ± 5 ng total RNA) significantly promoted IPEC-J2 cell proliferation by MTT, CCK8, EdU fluorescence and EdU flow cytometry assays. The qRT-PCR and Western blot analyses indicated milk-derived exosomes (0.27 ± 0.03 μg total RNA) significantly promoted expression of CDX2, IGF-1R and PCNA, and inhibited p53 gene expression involved in intestinal proliferation. Additionally, six detected miRNAs were significantly increased in IPEC-J2 cell, while FAS and SERPINE were significantly down-regulated relative to that in control. In vivo , treated groups (0.125 μg and 0.25 μg total RNA) significantly raised mice’ villus height, crypt depth and ratio of villus length to crypt depth of intestinal tissues, significantly increased CDX2, PCNA and IGF-1R’ expression and significantly inhibited p53′ expression. Our study demonstrated that milk-derived exosomes can facilitate intestinal cell proliferation and intestinal tract development, thus giving a new insight for milk nutrition and newborn development and health.