Open AccessPhysiological basis for low-temperature survival and storage of quiescent larvae of the fruit fly Drosophila melanogaster
Author(s) -
Vladimı́r Košťál,
Jaroslava Korbelová,
Tomáš Štětina,
Rodolphe Poupardin,
Hervé Colinet,
Helena Zahradníčková,
Iva Opekarová,
Martin Moos,
Petr Šimek
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep32346
Subject(s) - drosophila melanogaster , larva , melanogaster , biology , oxidative damage , transcriptome , cryopreservation , drosophila (subgenus) , metabolomics , microbiology and biotechnology , embryo , ecology , bioinformatics , oxidative stress , biochemistry , gene , gene expression
The cryopreservation techniques proposed for embryos of the fruit fly Drosophila melanogaster are not yet ready for practical use. Alternative methods for long-term storage of D. melanogaster strains, although urgently needed, do not exist. Herein, we describe a narrow interval of low temperatures under which the larvae of D. melanogaster can be stored in quiescence for up to two months. The development of larvae was arrested at the pre-wandering stage under fluctuating thermal regime (FTR), which simultaneously resulted in diminishing the accumulation of indirect chill injuries. Our physiological, metabolomic, and transcriptomic analyses revealed that compared to larvae stored at constant low temperatures, the larvae stored under FTR conditions were able to decrease the rates of depletion of energy substrates, exploited brief warm episodes of FTR for homeostatic control of metabolite levels, and more efficiently exerted protection against oxidative damage.