Open AccessImmunogenicity of a novel tetravalent vaccine formulation with four recombinant lipidated dengue envelope protein domain IIIs in mice
Author(s) -
ChiShiun Chiang,
Chien-Hsiung Pan,
Meiyu Chen,
Chun-Hsiang Hsieh,
JyPing Tsai,
Hsueh-Hung Liu,
ShihJen Liu,
Pele Chong,
ChihHsiang Leng,
HsinWei Chen
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep30648
Subject(s) - dengue vaccine , immunogenicity , dengue virus , dengue fever , recombinant dna , virology , adjuvant , immune system , biology , serotype , viral envelope , virus , immunology , biochemistry , gene
We developed a novel platform to express high levels of recombinant lipoproteins with intrinsic adjuvant properties. Based on this technology, our group developed recombinant lipidated dengue envelope protein domain IIIs as vaccine candidates against dengue virus. This work aims to evaluate the immune responses in mice to the tetravalent formulation. We demonstrate that 4 serotypes of recombinant lipidated dengue envelope protein domain III induced both humoral and cellular immunity against all 4 serotypes of dengue virus on the mixture that formed the tetravalent formulation. Importantly, the immune responses induced by the tetravalent formulation in the absence of the exogenous adjuvant were functional in clearing the 4 serotypes of dengue virus in vivo . We affirm that the tetravalent formulation of recombinant lipidated dengue envelope protein domain III is a potential vaccine candidate against dengue virus and suggest further detailed studies of this formulation in nonhuman primates.