Open AccessEnhanced biglycan gene expression in the adipose tissues of obese women and its association with obesity-related genes and metabolic parameters
Author(s) -
Jimin Kim,
Seul Ki Lee,
Jaehyun Shin,
Unwoo Jeoun,
Yeon Jin Jang,
Hye Soon Park,
Jong-Hyeok Kim,
Gyungyub Gong,
Taik Jong Lee,
Joon Pio Hong,
Yeon Ji Lee,
Yoon-Suk Heo
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep30609
Subject(s) - biglycan , adipose tissue , endocrinology , medicine , adipogenesis , adipose tissue macrophages , proinflammatory cytokine , extracellular matrix , inflammation , biology , white adipose tissue , microbiology and biotechnology , proteoglycan , decorin
Extracellular matrix (ECM) remodeling dynamically occurs to accommodate adipose tissue expansion during obesity. One non-fibrillar component of ECM, biglycan, is released from the matrix in response to tissue stress; the soluble form of biglycan binds to toll-like receptor 2/4 on macrophages, causing proinflammatory cytokine secretion. To investigate the pattern and regulatory properties of biglycan expression in human adipose tissues in the context of obesity and its related diseases, we recruited 21 non-diabetic obese women, 11 type 2 diabetic obese women, and 59 normal-weight women. Regardless of the presence of diabetes, obese patients had significantly higher biglycan mRNA in both visceral and subcutaneous adipose tissue. Biglycan mRNA was noticeably higher in non-adipocytes than adipocytes and significantly decreased during adipogenesis. Adipose tissue biglycan mRNA positively correlated with adiposity indices and insulin resistance parameters; however, this relationship disappeared after adjusting for BMI. In both fat depots, biglycan mRNA strongly correlated with the expression of genes related to inflammation and endoplasmic reticulum stress. In addition, culture of human preadipocytes and differentiated adipocytes under conditions mimicking the local microenvironments of obese adipose tissues significantly increased biglycan mRNA expression. Our data indicate that biglycan gene expression is increased in obese adipose tissues by altered local conditions.