Open AccessIsolation and characterization of a bacteriophage phiEap-2 infecting multidrug resistant Enterobacter aerogenes
Author(s) -
Erna Li,
Xiao Wei,
Yanyan Ma,
Zhe Yin,
Huan Li,
Weishi Lin,
Xuesong Wang,
Chao Li,
Zhiqiang Shen,
Rong Zhao,
Huiying Yang,
Aimin Jiang,
Wenhui Yang,
Jing Yuan,
Xiangna Zhao
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep28338
Subject(s) - enterobacter aerogenes , bacteriophage , siphoviridae , microbiology and biotechnology , biology , phage therapy , multiple drug resistance , genome , lytic cycle , virology , serratia , whole genome sequencing , gene , escherichia coli , genetics , bacteria , drug resistance , pseudomonas , virus
Enterobacter aerogenes (Enterobacteriaceae) is an important opportunistic pathogen that causes hospital-acquired pneumonia, bacteremia, and urinary tract infections. Recently, multidrug-resistant E. aerogenes have been a public health problem. To develop an effective antimicrobial agent, bacteriophage phiEap-2 was isolated from sewage and its genome was sequenced because of its ability to lyse the multidrug-resistant clinical E. aerogenes strain 3-SP. Morphological observations suggested that the phage belongs to the Siphoviridae family. Comparative genome analysis revealed that phage phiEap-2 is related to the Salmonella phage FSL SP-031 (KC139518). All of the structural gene products (except capsid protein) encoded by phiEap-2 had orthologous gene products in FSL SP-031 and Serratia phage Eta (KC460990). Here, we report the complete genome sequence of phiEap-2 and major findings from the genomic analysis. Knowledge of this phage might be helpful for developing therapeutic strategies against E. aerogenes .