Open AccessDiverse Genetic Background of Multidrug-Resistant Pseudomonas aeruginosa from Mainland China and Emergence of an Extensively Drug-Resistant ST292 Clone in Kunming
Author(s) -
Xin Fan,
Yue Wu,
Meng Xiao,
Zhi Peng Xu,
Timothy Kudinha,
Alda Bazaj,
Fanrong Kong,
Ying Xü
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep26522
Subject(s) - multilocus sequence typing , pulsed field gel electrophoresis , genotyping , biology , microbiology and biotechnology , outbreak , multiple drug resistance , molecular epidemiology , pseudomonas aeruginosa , genotype , typing , drug resistance , virology , genetics , gene , bacteria
For a better understanding of the multidrug resistant Pseudomonas aeruginosa (MDR-PA) epidemiology in mainland China, a nationwide surveillance network of 27 tertiary hospitals was established. Non-duplicate MDR-PA isolates from 254 cases of nosocomial infections, were collected during the period August 2011 to July 2012. Minimum inhibitory concentrations (MICs) of nine antimicrobial agents were determined by broth micro-dilution method according to the CLSI guidelines [M7-A10]. Genotyping analysis was performed by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). The presence of acquired carbapenemases was also determined by molecular approaches for 233 carbapenem-resistant isolates. Carbapenemase genes were detected in 19 (8.2%) isolates, with 13 of these isolates encoding IMP-type enzymes, five with VIM-2, and one with KPC-2. MLST analysis revealed significant genetic diversity among the MDR-PA isolates studied, and 91 STs (including 17 novel STs) were identified. However, a long-term outbreak of an emerging extensively drug-resistant (XDR) ST292/PFGE genotype A clone was detected in a hospital from Southwest China. This study has demonstrated that MDR-PA in mainland China have evolved from diverse genetic backgrounds. Evidence of clonal dissemination of the organism and nosocomial outbreaks in some regions, suggest a need to strengthen existing infection control measures.