Open AccessL-proline: a highly effective cryoprotectant for mouse oocyte vitrification
Author(s) -
Lu Zhang,
Xiaoyu Xue,
Jie Yan,
Liying Yan,
Xiaohu Jin,
Xiaohui Zhu,
ZhiZhu He,
Jing Liu,
Rong Li,
Jie Qiao
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep26326
Subject(s) - cryoprotectant , vitrification , oocyte , oocyte cryopreservation , cryopreservation , proline , andrology , biology , chemistry , microbiology and biotechnology , biochemistry , embryo , fertility preservation , medicine , amino acid , fertility , population , environmental health
Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian oocyte vitrification. In this study we evaluated the efficiency and safety of the new cryoprotectant in oocyte vitrification. The results indicated that L-proline improves the survival rate of vitrified oocytes, protects mitochondrial functions and could be applied as a new cryoprotectant in mouse oocyte vitrification.