Open AccessStable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
Author(s) -
Hideki Endo,
Megumi Yoshida,
Toshiki Uji,
Naotsune Saga,
Koji Inoue,
Hiromichi Nagasawa
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep22252
Subject(s) - transformation (genetics) , green fluorescent protein , polyethylene glycol , biology , gene , hygromycin b , peg ratio , botany , genetics , biochemistry , finance , economics
Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae , by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum.