Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae | Zendy

Hirotoshi Endo | Zendy; Megumi Yoshida | Zendy; Toshiki Uji | Zendy; Naotsune Saga | Zendy; Koji Inoue | Zendy; Hiromichi Nagasawa | Zendy

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Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae

Author(s) -

Hirotoshi Endo,

Megumi Yoshida,

Toshiki Uji,

Naotsune Saga,

Koji Inoue,

Hiromichi Nagasawa

Publication year - 2016

Publication title -

scientific reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.24

H-Index - 213

ISSN - 2045-2322

DOI - 10.1038/srep22252

Subject(s) - transformation (genetics) , green fluorescent protein , polyethylene glycol , biology , gene , hygromycin b , peg ratio , botany , genetics , biochemistry , finance , economics

Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae , by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum.

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