Open AccessInduction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9
Author(s) -
Junfeng Jiang,
Li Zhang,
Xingliang Zhou,
Xi Chen,
G. Huang,
Fengsheng Li,
Ruizhe Wang,
Nancy Wu,
Youzhen Yan,
Chang Tong,
Sankalp Srivastava,
Yue Wang,
Houqi Liu,
QiLong Ying
Publication year - 2016
Publication title -
scientific reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.24
H-Index - 213
ISSN - 2045-2322
DOI - 10.1038/srep21918
Subject(s) - crispr , chromosomal translocation , chromosomal abnormality , chromosome engineering , biology , embryonic stem cell , cas9 , genetics , stem cell , microbiology and biotechnology , chromosome , karyotype , gene
Chromosomal translocation is the most common form of chromosomal abnormality and is often associated with congenital genetic disorders, infertility, and cancers. The lack of cellular and animal models for chromosomal translocations, however, has hampered our ability to understand the underlying disease mechanisms and to develop new therapies. Here, we show that site-specific chromosomal translocations can be generated in mouse embryonic stem cells (mESCs) via CRISPR/Cas9. Mouse ESCs carrying translocated chromosomes can be isolated and expanded to establish stable cell lines. Furthermore, chimeric mice can be generated by injecting these mESCs into host blastocysts. The establishment of ESC-based cellular and animal models of chromosomal translocation by CRISPR/Cas9 provides a powerful platform for understanding the effect of chromosomal translocation and for the development of new therapeutic strategies.