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Herpesvirus saimiri transformation may help disclose inherent functional defects of mucosal T lymphocytes in patients with gastric adenocarcinoma
Author(s) -
Valeri Anna P,
AguileraMontilla Noemí,
LópezSantalla Mercedes,
Mencía Ángeles,
RodríguezJuan Cristina,
GutiérrezCalvo Alberto,
Martín Javer,
Lasa Inmaculada,
GarcíaSancho Luis,
Granell Javier,
PérezBlas Mercedes,
MartínVilla José M
Publication year - 2008
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/sj.icb.7100157
Subject(s) - cd3 , adenocarcinoma , monoclonal antibody , cancer , microbiology and biotechnology , biology , immunology , antibody , medicine , gastroenterology , antigen , cancer research , cd8
To dissect the phenotypic and functional features of mucosal T lymphocytes in patients with gastric adenocarcinoma, we have used the Herpesvirus saimiri transformation procedure to achieve T‐cell lines from gastric origin. Once achieved, cell function was assessed by in vitro stimulation with mitogens. CD2‐specific monoclonal antibodies (α‐CD2), alone or in combination with interleukin (IL)‐2, rendered fewer counts in patients (34 408±3965 and 52 157±6473 c.p.m., respectively) than in controls (67 471±11 755 c.p.m., P <0.01 and 77 864±12 545 c.p.m., P <0.05, respectively). Likewise, CD3‐based responses were defective in cancer cell lines: α‐CD3 (54 794±9269 vs 86 104±10 341 c.p.m., P <0.01), α‐CD3+IL‐2 (57 789±8590 vs 88855±8516 c.p.m., P <0.01) and α‐CD3+α‐CD2 (52 130±7559 vs 120 852±16 552 c.p.m., P <0.01). Finally, IL‐2 failed to adequately stimulate patient cell lines (39 310±4023 vs 60 945±9463 c.p.m., P <0.05). These results suggest that mucosal T lymphocytes in cancer patients are inherently impaired in their proliferative ability. This may be crucial in the control of tumour growth.