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Monocyte‐derived dendritic cells from chagasic patients vs healthy donors secrete differential levels of IL‐10 and IL‐12 when stimulated with a protein fragment of Trypanosoma cruzi heat‐shock protein‐70
Author(s) -
Cuellar Adriana,
Santander Sandra Paola,
Thomas María del Carmen,
Guzmán Fanny,
Gómez Alberto,
López Manuel Carlos,
Puerta Concepción J
Publication year - 2008
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/sj.icb.7100146
Subject(s) - cd86 , trypanosoma cruzi , secretion , peripheral blood mononuclear cell , tumor necrosis factor alpha , biology , immune system , immunology , chagas disease , antigen , dendritic cell , monocyte , heat shock protein , t cell , parasite hosting , endocrinology , in vitro , gene , biochemistry , world wide web , computer science
We analyzed the effect of the truncated heat‐shock protein 70 from Trypanosoma cruzi on maturation of human dendritic cells (DCs) derived from monocytes of peripheral blood mononuclear cells from healthy donors and chagasic patients. The results show that the T‐HSP70 is capable of maturing human DCs inducing an increase in the expression level of the CD83, CD86 and human leukocyte antigen‐DR surface markers, as well as in the secretion of interleukin (IL)‐12, tumor necrosis factor‐α (TNF‐α) and IL‐6 cytokines. Results also show the existence of a differential functional activity of matured DCs from chagasic patients vs healthy donors in response to T‐HSP70 protein and to HSP‐70‐derived A72 peptide, as only T‐HSP70‐matured DCs from chagasic patients have an enhanced secretion of IL‐10 and a reduced secretion of IL‐12. Moreover, the addition of A72 peptide to immature DCs from chagasic patients induced an increase in the percentage of cells expressing CD83 and CD86 molecules regarding to the expression level observed by cells from healthy donors. These findings suggest that T. cruzi HSP70 protein may induce a specific maturation profile on chagasic patients' DCs, which would favor the persistence of the parasite in the human host.