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The regulation of the B‐cell gene expression programme by Pax5
Author(s) -
Holmes Melissa L,
Pridans Clare,
Nutt Stephen L
Publication year - 2008
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/sj.icb.7100134
Subject(s) - pax5 , b cell , biology , lymphopoiesis , gene , transcription factor , gene expression , regulation of gene expression , microarray analysis techniques , gene expression profiling , genetics , microbiology and biotechnology , antibody , stem cell , haematopoiesis
The activity of the transcription factor paired box gene 5 (Pax5) is essential for many aspects of B lymphopoiesis including the initial commitment to the lineage, immunoglobulin rearrangement, pre‐B cell receptor signalling and maintaining cell identity in mature B cells. Deregulated or reduced Pax5 activity has also been implicated in B‐cell malignancies both in human disease and mouse models. Candidate gene approaches and biochemical analysis have revealed that Pax5 regulates B lymphopoiesis by concurrently activating B cell‐specific gene expression as well as repressing the expression of genes, many of which are associated with non‐B cell lineages. These studies have been recently complemented with more exhaustive microarray studies, which have identified and validated a large panel of Pax5 target genes. These target genes reveal a gene regulatory network, with Pax5 at its centre that controls the B‐cell gene expression programme.