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A critical role for CCL2 and CCL3 chemokines in the regulation of polymorphonuclear neutrophils recruitment during corneal infection in mice
Author(s) -
Xue MeiLang,
Thakur Archana,
Cole Nerida,
Lloyd Andrew,
Stapleton Fiona,
Wakefield Denis,
Willcox Mark D P
Publication year - 2007
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/sj.icb.7100082
Subject(s) - ccl3 , chemokine , immunology , chemotaxis , cornea , inflammation , ccl2 , infiltration (hvac) , keratitis , microbiology and biotechnology , biology , medicine , receptor , physics , genetics , neuroscience , thermodynamics
While the role of CC chemokines in mononuclear cell trafficking and activation has been well studied, the functional role of CC chemokines in the regulation of polymorphonuclear neutrophil (PMN) recruitment in vivo has not been widely examined. Bacterial infection of the cornea (keratitis) is a relatively common, sometimes sight‐threatening disease, which features acute inflammation with ulceration and PMN infiltration. Here, we demonstrate a critical role for the chemokines, CCL2 and CCL3, in the Pseudomonas aeruginosa‐ induced model of corneal infection in BALB/c mice. Treatment of mice with anti‐CCL2 or anti‐CCL3 antibodies resulted in a significant reduction in severity of corneal damage and PMN infiltration at 1 and 7 days after infection compared to control antibody‐treated eyes, but did not significantly alter the rate of bacterial clearance from the cornea. Our findings provide strong evidence that CCL2 and CCL3 are critical regulators of PMN recruitment, and may lead to therapeutic strategies via targeting of the CC chemokines, CCL2 and CCL3, in the management of P. aeruginosa keratitis.