Premium
Anti‐BDCA‐4 (neuropilin‐1) antibody can suppress virus‐induced IFN‐alpha production of plasmacytoid dendritic cells
Author(s) -
GrageGriebenow Evelin,
Löseke Stefan,
Kauth Marion,
Gehlhar Kirsten,
Zawatzky Rainer,
Bufe Albrecht
Publication year - 2007
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/sj.icb.7100048
Subject(s) - antibody , virology , alpha (finance) , plasmacytoid dendritic cell , virus , chemistry , biology , microbiology and biotechnology , immunology , dendritic cell , medicine , immune system , construct validity , nursing , patient satisfaction
Plasmacytoid dendritic cells (PDC) in human blood are the main source of virus‐induced interferon (IFN)‐alpha. They exhibit a lineage‐negative phenotype but all express BDCA‐4, which is homologous to the neuronal receptor neuropilin‐1. Specific staining with anti‐BDCA‐4 antibody is used for positive isolation of PDC from blood by magnetic cells sorting. Here, it is demonstrated that these positively selected PDC showed reduced or completely abolished IFN‐alpha release compared to unstained PDC, which were negatively selected by magnetic depletion of lineage‐positive blood mononuclear cells. In addition, treatment of these unstained PDC with anti‐BDCA‐4 mAb also resulted in at least two‐fold lower or reduced virus‐induced IFN‐alpha production. It is shown that the antibody not only affects cell survival or block virus attachment but also reduces IFN‐alpha release induced by non‐viral CpG oligodeoxynucleotides. In conclusion, data suggest an immunoregulatory role for BDCA‐4 on PDC as demonstrated for IFN‐alpha response to virus.