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Adenovirus protein IX sequesters host‐cell promyelocytic leukaemia protein and contributes to efficient viral proliferation
Author(s) -
RosaCalatrava Manuel,
PuvionDutilleul Francine,
Lutz Pierre,
Dreyer Dominique,
De Thé Hugues,
Chatton Bruno,
Kedinger Claude
Publication year - 2003
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.embor943
Subject(s) - biology , promyelocytic leukemia protein , capsid , virology , viral protein , adenoviridae , cell growth , nuclear protein , microbiology and biotechnology , virus , gene , genetic enhancement , transcription factor , genetics
The product of adenovirus type 5 (Ad5) gene IX, protein IX (pIX), is a multifunctional protein that stabilizes the viral capsid and has transcriptional activity. We show that pIX also contributes to the Ad5‐induced reorganization of the host‐cell nuclear ultrastructure: pIX induces the formation of specific and dynamic nuclear inclusions, and the host promyelocytic leukaemia (PML) protein, which is the main structural organizer of PML bodies, is stably relocated and confined within the pIX‐induced inclusions late in infection. Our results suggest that Ad5 has evolved a unique strategy that leads to the sustained neutralization of PML bodies throughout infection, thereby ensuring optimal viral proliferation.