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Multiple roles for kinases in DNA replication
Author(s) -
Henneke Ghislaine,
Koundrioukoff Stéphane,
Hübscher Ulrich
Publication year - 2003
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.embor774
Subject(s) - replisome , eukaryotic dna replication , origin recognition complex , pre replication complex , replication factor c , dna replication , control of chromosome duplication , dna replication factor cdt1 , microbiology and biotechnology , biology , dna re replication , phosphorylation , proliferating cell nuclear antigen , dna , genetics
DNA replication is carried out by the replisome, which includes several proteins that are targets of cell‐cycle‐regulated kinases. The phosphorylation of proteins such as replication protein A, DNA polymerase‐α and ‐δ, replication factor C, flap endonuclease 1 and DNA ligase I leads to their inactivation, suggesting that phosphorylation is important in the prevention of re‐replication. Moreover, the phosphorylation of several of these replication proteins has been shown to block their association with the ‘moving platform’—proliferating cell nuclear antigen. Therefore, phosphorylation seems to be a crucial regulator of replisome assembly and DNA replication, although its precise role in these processes remains to be clarified.