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New androgen receptor genomic targets show an interaction with the ETS1 transcription factor
Author(s) -
Massie Charles E,
Adryan Boris,
BarbosaMorais Nuno L,
Lynch Andy G,
Tran Maxine G,
Neal David E,
Mills Ian G
Publication year - 2007
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7401046
Subject(s) - androgen receptor , transcription factor , ets1 , biology , genetics , computational biology , transcription (linguistics) , microbiology and biotechnology , gene , prostate cancer , cancer , linguistics , philosophy
The androgen receptor (AR) initiates important developmental and oncogenic transcriptional pathways. The AR is known to bind as a homodimer to 15‐base pair bipartite palindromic androgen‐response elements; however, few direct AR gene targets are known. To identify AR promoter targets, we used chromatin immunoprecipitation with on‐chip detection of genomic fragments. We identified 1,532 potential AR‐binding sites, including previously known AR gene targets. Many of the new AR target genes show altered expression in prostate cancer. Analysis of sequences underlying AR‐binding sites showed that more than 50% of AR‐binding sites did not contain the established 15 bp AR‐binding element. Unbiased sequence analysis showed 6‐bp motifs, which were significantly enriched and were bound directly by the AR in vitro . Binding sequences for the avian erythroblastosis virus E26 homologue (ETS) transcription factor family were also highly enriched, and we uncovered an interaction between the AR and ETS1 at a subset of AR promoter targets.