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Structural and functional analysis of RNA and TAP binding to SF2/ASF
Author(s) -
Tintaru Aura M,
Hautbergue Guillaume M,
Hounslow Andrea M,
Hung MingLung,
Lian LuYun,
Craven C Jeremy,
Wilson Stuart A
Publication year - 2007
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7401031
Subject(s) - rna , computational biology , biology , genetics , chemistry , gene
The serine/arginine‐rich (SR) protein splicing factor 2/alternative splicing factor (SF2/ASF) has a role in splicing, stability, export and translation of messenger RNA. Here, we present the structure of the RNA recognition motif (RRM) 2 from SF2/ASF, which has an RRM fold with a considerably extended loop 5 region, containing a two‐stranded β‐sheet. The loop 5 extension places the previously identified SR protein kinase 1 docking sequence largely within the RRM fold. We show that RRM2 binds to RNA in a new way, by using a tryptophan within a conserved SWQLKD motif that resides on helix α1, together with amino acids from strand β2 and a histidine on loop 5. The linker connecting RRM1 and RRM2 contains arginine residues, which provide a binding site for the mRNA export factor TAP, and when TAP binds to this region it displaces RNA bound to RRM2.