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Rmi1, a member of the Sgs1–Top3 complex in budding yeast, contributes to sister chromatid cohesion
Author(s) -
Lai Mong Sing,
Seki Masayuki,
Ui Ayako,
Enomoto Takemi
Publication year - 2007
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7401000
Subject(s) - establishment of sister chromatid cohesion , genome instability , biology , sister chromatids , saccharomyces cerevisiae , genetics , dna damage , mutant , microbiology and biotechnology , rad51 , dna , yeast , gene , chromosome
The Saccharomyces cerevisiae RecQ‐mediated genome instability (Rmi1) protein was recently identified as the third member of the slow growth suppressor 1–DNA topoisomerase III (Sgs1–Top3) complex, which is required for maintaining genomic stability. Here, we show that cells lacking RMI1 have a mitotic delay, which is partly dependent on the spindle checkpoint, and are sensitive to the microtubule depolymerizing agent benomyl. We show that rmi1 and top3 single mutants are defective in sister chromatid cohesion, and that deletion of SGS1 suppresses benomyl sensitivity and the cohesion defect in these mutant cells. Loss of RAD51 also suppresses the cohesion defect of rmi1 mutant cells. These results indicate the existence of a new pathway involving Rad51 and Sgs1‐Top3‐Rmi1, which leads to the establishment of sister chromatid cohesion.