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MEL‐28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly
Author(s) -
Franz Cerstin,
Walczak Rudolf,
Yavuz Sevil,
Santarella Rachel,
Gentzel Marc,
Askjaer Peter,
Galy Vincent,
Hetzer Martin,
Mattaj Iain W,
Antonin Wolfram
Publication year - 2007
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1038/sj.embor.7400889
Subject(s) - nuclear pore , nucleoporin , microbiology and biotechnology , chromatin , mitosis , biology , interphase , caenorhabditis elegans , nuclear transport , cell nucleus , nucleus , genetics , dna , gene
The metazoan nuclear envelope (NE) breaks down and re‐forms during each cell cycle. Nuclear pore complexes (NPCs), which allow nucleocytoplasmic transport during interphase, assemble into the re‐forming NE at the end of mitosis. Using in vitro NE assembly, we show that the vertebrate homologue of MEL‐28 (maternal effect lethal), a recently discovered NE component in Caenorhabditis elegans , functions in postmitotic NPC assembly. MEL‐28 interacts with the Nup107–160 complex (Nup for nucleoporin), an important building block of the NPC, and is essential for the recruitment of the Nup107–160 complex to chromatin. We suggest that MEL‐28 acts as a seeding point for NPC assembly.